Catalog Number - 85518-10P-2

The 85518‑10P‑2 Cogent RP C18 ABX™ HPLC Column is engineered for durable, reproducible separations in extreme mobile phase conditions, delivering stable performance across a wide pH range (2–12).

Built with a bulky, specialized end‑capped C18 phase bonded to high‑purity silica, this column minimizes secondary interactions and enhances peak shape for acidic, basic, and neutral compounds, including those with very high or very low pKa values.

✅ Ideal as a USP L1 alternate for demanding acid/base methods

100 mm Length – Increased Resolution for LC‑MS Methods

• Higher resolving power than 50 mm columns

• Improves separation of closely related analytes

• Increases peak capacity for complex mixtures

• Ideal for method refinement after initial screening

✅ Selected when greater resolution is required beyond fast scouting workflows

2.1 mm ID – LC‑MS / Low‑Flow Optimization

• Designed for low‑flow HPLC and LC‑MS systems

• Enhances MS sensitivity through reduced solvent load

Typical Flow Rate Range

• ~0.2–0.3 mL/min

✅ Requires precise low‑flow pump delivery and low‑volume flow cells

⚠️ Narrow‑bore format requires stable flow control for optimal performance

5 µm Particle – Maximum Robustness & Method Transfer

• Provides:

  • Excellent durability and long column life

  • Lower backpressure vs smaller particle sizes

  • Consistent, reproducible performance

✅ Ideal for:

• Routine LC‑MS workflows

• Method transfer across instruments and labs

• Systems with pressure limitations

Wide pH Stability (pH 2–12) – Extreme Method Capability

• Designed for acidic and basic mobile phases

• Suitable for:

  • Strong acids

  • Strong bases

• Maintains integrity under aggressive analytical conditions

✅ Enables separations beyond standard C18 limitations

Why Choose RP C18 ABX™ Columns (5 µm)

Extreme pH Ruggedness

• Stable across full pH range (2–12)

• Ideal for stability‑indicating and harsh methods

Maximum Durability & Consistency

• Designed for long‑term use and reproducibility

• Supports routine QC and validated methods

Versatile Analytical Performance

• Suitable for:

  • Hydrophobic compounds

  • Neutral analytes

  • Long‑chain fatty acids

✅ One of the most reliable ABX options for routine LC‑MS workflows

LC‑MS Workflow Advantages

• 2.1 mm format:

  • Improves detection sensitivity

  • Reduces solvent consumption

• 100 mm length:

  • Provides enhanced resolution vs short columns

  • Improves method robustness for complex samples

✅ Ideal for balanced LC‑MS workflows requiring durability and resolution

System & Sample Consideration

• Designed for low‑flow LC‑MS and analytical HPLC systems

• Requires:

  • Accurate low‑flow pump capability

  • Low‑volume detector flow cells

Recommended sample preparation:

• 👉 Use 0.2 µm syringe filters for proper sample preparation before injection

✅ Helps:

• Prevent column fouling and blockage

• Extend column lifetime and performance

Typical Applications

• LC‑MS and LC‑MS/MS analysis

• Acidic and basic compound separations

• Pharmaceutical QC and validation

• Stability‑indicating methods

• Routine analytical workflows

• Complex mixture separations

Important Usage Notes

• Designed for 2.1 mm LC‑MS and low‑flow systems (~0.2–0.3 mL/min)

• Suitable for extreme pH mobile phase conditions (pH 2–12)

• Ideal for routine and high‑reliability workflows

• Confirm system compatibility for low‑flow precision